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ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. Overcentrifugation may cause either a false negative result (if too much agitation is required for resuspension), or a false positive, (if centrifuged clumps cannot be completely dispersed). High concentration of IgG paraprotein, and failure to adequately wash cells can leave unbound IgG which will neutralize antiglobulin reagent. Delay of addition of antiglobulin reagent may allow previously bound IgG antibody to dissociate from red cells.
Blood bank
Which of the following might cause a false positive indirect antiglobulin test:

A) High concentration of IgG paraprotein in a patient's serum
B) Delay of addition of antiglobulin reagent.
C) Overcentrifugation
D) Failure to adequately wash cells

2. Animals serve as a reservoir of infection" is the correct answer because Shigella only infects humans while Salmonella is found in many animals. Both have diarrhea as the major symptom. Endotoxin is produced by all gram negative bacteria and exotoxin is only produced by Shigella.
A major difference between Salmonella enterica and Shigella infections is that only in salmonellosis can:

A) endotoxin play a role in disease process
B) exotoxin produce profuse watery stools
C) diarrhea become the major symptom
D) animals serve as a reservoir of infection

3. Ammonium biurate crystalsare typically round, irregularly spiked and yellow-brown in color.
A microscopic examination of a normal urine pH 8.0 shows 2+ yellow-brown thorny spheres which are MOST probably:

A) waxy casts
B) amorphous urate crystals
C) crenated red cells
D) ammonium biurate crystals
E) ampicillin crystals

4. Vitamin K dependent factors are those that require Vitamin K for their synthesis in the liver. Vitamin K is an important factor to gamma-glutamyl carboxylase which adds a carboxyl group to glutamic acid residues on factors II, VII, IX and X, as well as Protein S, Protein C. In adding the gamma-carboxyl group to glutamate residues on the immature clotting factors Vitamin K is itself oxidized. Deficiency of Vitamin K due to malabsorption, liver disease, etc. may contribute to bleeding disorders because clotting factor maturation depends on Vitamin K.
Which of these coagulation factors are referred to as "vitamin-K dependent?"

A) II, V, IX, XII
B) I, V, VIII, XIII
C) II, VII, IX, X
D) XI, XII, Fletcher, Fitzgerald

5. Modified Thayer Martin agar at 35ºC with CO2 is the correct answer. This is a typical presentation of systemic gonorrhea and Neisseria gonorrhea grows best on MTM medium at 35ºC. under CO2. Systemic gonorrhea occurs in 1% of cases and results in purulent arthritis and rarely septicemia.
An 18-year-old woman woke up in the morning to find that her left knee was hot, swollen, and very painful.
She could not walk to work and a friend offered her a ride to the clinic. She had never had a swollen joint before. Physical exam revealed a tender knee joint which yielded purulent synovial fluid on aspiration.
Gram-negative intracellular diplococci were seen on Gram stain. She also had a cervical discharge that was cultured. The cause of her infection would grow best on:

A) LIM (Todd Hewitt Broth w/Colistin and Nalidixic Acid) broth with refrigerated temperature
B) V (human blood) agar at 35ºC with CO2
C) Modified Thayer Martin agar at 35ºC with CO2
D) Blood agar at 42ºC with microaerophiilic conditions

Solutions: